5-(6-(6-(6-(4-N-(3-Dimethoxytrityloxypropyl)-N-(4'-nitro-2'-chloroazobenzen-4-yl)-aminobutanoyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carbonyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carbonyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carboxamido)pentyl-1-O-diglycoloyl long chain alkylamino CPG
MGB Eclipse® pairs a minor groove binding (MGB) tripeptide with a dark quencher. The MGB significantly enhances hybridization while the Eclipse® has a broad absorption range that quenches many of the most common fluorophores including FAM, HEX, TET and Yakima Yellow®. Together, MGB Eclipse® is an attractive group for the synthesis of hydrolysis probes, the most common probe detection method for qPCR.
Details
Usage
Coupling: Regular with UltraMild monomers and Cap A (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx and Cap A 40-4210-xx/40-4212-xx). Use 0.5 M CSO in ACN for oxidation (Catalog Number 40-4632-xx) using a 3 minute oxidation time.
Deprotection: Cleave and deprotect the oligonucleotide in 30% Ammonium Hydroxide for 2 hours at Room Temperature. If standard monomers and iodine oxidation are used, acceptable results can be obtained when deprotected in NH4OH/EtOH 3:1 (v/v) for 17 hr at 55°C.
Specifications
Storage
Freezer storage, -10 to -30°C, dry
Intellectual Property
Yakima Yellow®, Redmond Red®, Eclipse® and MGB Eclipse® are registered Trademarks of ELITechGroup. Product is made and sold under license from ELITechGroup.
